Cloning of Major Surface Antigen (SAG1) Gene of Toxoplasma gondii Tachyzoites
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چکیده
منابع مشابه
Cloning of Major Surface Antigen (SAG1) Gene of Toxoplasma gondii Tachyzoites
Toxoplasma gondii is an obligate intracellular protozoon parasite of all the warm blooded animals including birds and humans. It completes a sexual part of its life cycle in the intestinal epithelial cell of the Felidae (definitive host) and an asexual part in warm blooded (intermediate host). Human is an accidental host who contracts infection through handling cat litter and gardening, ingesti...
متن کاملCloning and sequencing of Toxoplasma gondii major surface antigen (SAG1) gene
Genetic typing methods of T. gondii strains have been extensively perfected in recent years. From a technical point of view, many tools usable for genetic studied on single-copy loci have been used: RFLP, PCR-RFLP, sequencing, RAPD-PCR and isoenzyme analysis. We described the cloning and sequence analysis of the gene which encodes the major surface antigen (SAG1 or P30) of T. gondii. SAG1 is ...
متن کاملcloning and sequencing of toxoplasma gondii major surface antigen (sag1) gene
genetic typing methods of t. gondii strains have been extensively perfected in recent years. from a technical point of view, many tools usable for genetic studied on single-copy loci have been used: rflp, pcr-rflp, sequencing, rapd-pcr and isoenzyme analysis. we described the cloning and sequence analysis of the gene which encodes the major surface antigen (sag1 or p30) of t. gondii. sag1 is th...
متن کاملgene cloning of 30 kda toxoplasma gondii tachyzoites surface an¬tigen (sag1)
background: toxoplasma gondii is an obligate intracellular parasite and its sexual and asexual cycles, respectively take place in the intestinal epithelial cell of definitive host and tissue of intermediate hosts. congenital toxoplasmosis is more impor tant when the mother acquired the infection during pregnancy period for the first time. serological tests are the only meth ods for diagnosis of...
متن کاملToxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding.
Previous studies have indicated that SAG1, the major surface molecule of the protozoan parasite Toxoplasma gondii, is an important attachment ligand for the host cell. However, the research data that supports this claim comes largely from studies investigating tachyzoite binding, and not SAG1 binding per se. In this study we successfully developed an in vitro attachment assay to directly evalua...
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ژورنال
عنوان ژورنال: International Journal of Current Microbiology and Applied Sciences
سال: 2017
ISSN: 2319-7692,2319-7706
DOI: 10.20546/ijcmas.2017.607.019